Enhancement of ethanol production using CO2and CO gases in a continuous fixed-film bioreactor
2
Issued Date
2025-07-01
Resource Type
ISSN
22129820
Scopus ID
2-s2.0-105009213976
Journal Title
Journal of Co2 Utilization
Volume
97
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Co2 Utilization Vol.97 (2025)
Suggested Citation
Potivichayanon S., Pokethitiyook P., Toensakes R. Enhancement of ethanol production using CO2and CO gases in a continuous fixed-film bioreactor. Journal of Co2 Utilization Vol.97 (2025). doi:10.1016/j.jcou.2025.103133 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/111069
Title
Enhancement of ethanol production using CO2and CO gases in a continuous fixed-film bioreactor
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Abstract
Ethanol-producing bacteria play an essential role in enhancing bioethanol production efficiency. This study aimed to develop an application of Petrobacter sp. SUTSP5, an ethanol-producing bacterium, from carbon-rich gas by using a fixed-film ethanol bioreactor called FEthaRex to enhance ethanol production efficiency. The fixed-film consortium was initially grown in a combination nutrient with a carbon-rich gas composed of 15:25:40 (by volume) CO2:CO:H2 and supplied to the FEthaRex column at an average flow rate of 100 mL/min. Nutritional liquid medium was circulated at a flow rate of 20 mL/min. The system achieved an ethanol production rate of approximately 0.1305 g/L/h within 24 h, which increased significantly to 0.1780 g/L/h after a 48-h operation period; the ethanol production rate increased from 0.1585-0.2132 g/L/h as the gas composition increased to 30:50:20 (by volume); continuous operation of the FEthaRex system was achieved after mixed gas feeding was stopped; and the maximum ethanol concentration observed after 48 h was 1.9860 g/L/h, or 6.0411 % by volume, with the ethanol-producing cells reaching a count of approximately 107 CFU/mL and average biofilm cell attached to packing media roughly 0.0058 g. At these optimum conditions, which achieved an ethanol yield higher than that of a free suspended cell reactor (EthaRex) with the same gas feed conditions. The FEthaRex system was demonstrated as a promising approach to achieving cell density and stability in the microbial community. This technology could be an efficient tool for enhancing sustainable bioethanol production.