Transcriptome Analysis of Monocytes Treated With Dengue Virus Nonstructural Protein 1 Revealed a Shift in Transcripts Involved in Self-Propagated Proinflammation and Antiviral Responses
Issued Date
2025-06-15
Resource Type
ISSN
00221899
eISSN
15376613
Scopus ID
2-s2.0-105010940659
Pubmed ID
40166903
Journal Title
Journal of Infectious Diseases
Volume
231
Issue
6
Start Page
e1170
End Page
e1182
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Infectious Diseases Vol.231 No.6 (2025) , e1170-e1182
Suggested Citation
Saisingha K., Jearanaiwitayakul T., Watterson D., Modhiran N., Ponpuak M., Ubol S. Transcriptome Analysis of Monocytes Treated With Dengue Virus Nonstructural Protein 1 Revealed a Shift in Transcripts Involved in Self-Propagated Proinflammation and Antiviral Responses. Journal of Infectious Diseases Vol.231 No.6 (2025) , e1170-e1182. e1182. doi:10.1093/infdis/jiaf166 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/111385
Title
Transcriptome Analysis of Monocytes Treated With Dengue Virus Nonstructural Protein 1 Revealed a Shift in Transcripts Involved in Self-Propagated Proinflammation and Antiviral Responses
Author's Affiliation
Corresponding Author(s)
Other Contributor(s)
Abstract
Nonstructural protein 1 (NS1) of dengue virus (DENV) can influence dengue severity. In this study, we used RNA sequencing analysis to assess the blood monocyte response to different concentrations of NS1. We showed that NS1 at the level found in severe dengue may be involved in severe dengue development through 2 potential mechanisms: induction of excessive inflammation and suppression of antiviral responses. At high levels, NS1 significantly up-regulated S100A8 and S100A9, ACOD1, and TREM1, which might help amplify the inflammatory loops. In terms of antiviral suppression, we found that high NS1 concentration significantly suppressed interferon signaling and major histocompatibililty complex class II transcripts. This potentially delayed the clearance of both DENV and NS1 protein. Our study highlighted the possible role of NS1-activated monocytes in dengue severity.