Journal Issue: JAAS Vol. 14 No. 1
1
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2564
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tha
eng
eng
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ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
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คณะสัตวแพทยศาสตร์ มหาวิทยาลัยมหิดล
Journal Volume
JAAS Volume 14
(2564)
Articles
Recombinant Proteins as Antigens for Serological Detection of Toxoplasma gondii and Neospora caninum in Livestock
(2021) Ruenruetai Udonsom; Supaluk Popruk; Charoonluk Jirapattharasate; Aongart Mahittikorn; รื่นฤทัย อุดรโสม; สุภลัคน์ โพธิ์พฤกษ์; จารุญลักษณ์ จิรภัทรเศรษฐ์; องอาจ มหิทธิกร; Mahidol University. Faculty of Tropical Medicine. Department of Protozoology; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied animal science,
Toxoplasmosis and neosporosis are diseases of livestock worldwide caused by infections with closely
related parasitic protozoa, T. gondii and N. caninum, respectively. Toxoplasmosis is a cause of reproductive
failure in small ruminants and zoonotic, while neosporosis is a major cause of bovine abortion without zoonotic
reports. The clinical signs associated with both infections are often nonspecific. Therefore, serological diagnosis is important for detection of specific antibodies induced by the infection. However, propagation of T. gondii and N. caninum tachyzoite in vitro or in vivo is required prior to crude antigen extraction, high risk in contamination of cell culture or animal facilities for parasite propagation and time-consuming process. With the use of recombinant proteins as antigens, the risk of handing viable parasites can be avoided with improving in sensitivity and specificity for the detection. Although some of T. gondii or N. caninum recombinant proteins showed a high efficacy for diagnosis, more validation and optimization are still needed to provide a high throughput performance for using in animals. This review presents advance in the application of recombinant antigens as a serological marker for the above parasites detection in livestock.
The Effects of Heartwood Extracts from Biancaea sappan L. against Coagulase-Negative Staphylococci (CoNS) and Staphylococcus aureus Isolated from Subclinical Mastitis in Dairy Goats
(2021) Panitnun Leelakhachonchit; Raweephun Phongprai; Wannapa Jiennumwong; Chowalit Nakthong; Norasuthi Bangphoomi; Arpron Leesombun; Orathai Thongjuy; Sookruetai Boonmasawai; Mahidol University. Faculty of Veterinary Science; Mahidol University. Faculty of Veterinary Science. The Center for Veterinary Diagnosis; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Science
Subclinical mastitis is an important disease affecting milk quality in dairy goats, and slow to detect because
of non-clinical signs. Subclinical mastitis will progress to clinical mastitis which requires the treatments. The
treatment mainly uses the antimicrobial intra-mammary infusion, and it may cause antimicrobial resistance
problems. Thus, this study aimed to determine the change of milk composition and the antibacterial activity of
ethanolic extracts from Biancaea sappan L. heartwood against bacteria isolated from milk of subclinical mastitis
goats. Eighty-eight milk samples were classified into two groups; control (n=34) and subclinical mastitis group
or SCM (n=54) according to California mastitis test (CMT) score and somatic cell count (SCC). The study showed
that the percentage of lactose significantly decreased (p=0.002) in the subclinical mastitis goats, whereas the fat
contents significantly increased (p=0.014). The changing of milk protein, solids not fat (SNF) and total solid (TS)
contents were not observed. The bacterial identifications from milk samples revealed that the most commonly
isolated bacteria were coagulase-negative staphylococci (CoNS) (n=41, 78.9%) and Staphylococcus aureus
(n=7, 13.5%). The ethanolic extracts from B. sappan showed their antibacterial activity against isolated CoNS
and S. aureus with minimum inhibitory concentration (MIC) range were 0.125-4 mg/ml and 0.125-1 mg/ml,
respectively. These findings indicated that B. sappan ethanolic extracts potentially effected against major
pathogens of subclinical mastitis in dairy goats
Using of CRFK and HeLa Dual Cell-Culture System to Isolate Canine Distemper Virus
(2020) Jarupha Taowan; Pruksa Julapanthong; Wanvisa Surarith; Natthapat Ketchim; Chalisa Mongkolphan; Kridsada Chaichoun; Mahidol University. Faculty of Veterinary Science; Mahidol University. Faculty of Veterinary Science. The Monitoring and Surveillance Center for Zoonotic Disease in Wildlife and Exotic Animals; Mahidol University. Faculty of Veterinary Science. Internal Medicine Unit Prasu Arthorn Animal Hospital
Canine distemper virus (CDV) is a common infectious agent that affects respiratory and nervous systems
in canine, pet and wildlife worldwide. The virus isolation using cell culture is one of laboratory diagnostic
methods for detection of virus infections. Many different types of cultured cells can be used for isolation of
distemper virus, which provides the different outcome. This study aimed to evaluate the efficacy of dual cell culture system, Crandell-Rees feline kidney (CRFK) and HeLa cells for virus isolation. A total of 13 clinical
specimens were taken by nasal and oropharynx swab from dogs with clinical signs compatible with CDV
infection. These specimens were positively confirmed CDV by using Reverse transcriptase - polymerase chain
reaction (RT-PCR) for viral genome detection. The CDV RT-PCR positive specimens were then taken to isolate
in both cell-lines for five passages, and were confirmed by RT-PCR again. By using CRFK and HeLa cells, the
successful viral isolation with CDV positive specimens was recorded at 7/13 (53.84%) in CRFK and 6/13 (46.15%) in HeLa cells, respectively. The dual cell-culture system showed an increase of the successful isolation at 8/13 (61.53%). Our results suggested that the application of dial cell-culture system was more effective than using single cell-culture for isolation of canine distemper virus.
Title
JAAS Vol. 14 No. 1
Author's Affiliation
มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์