Publication: Functional expression in Beauveria bassiana of a chitinase gene from Ophiocordyceps unilateralis, an ant-pathogenic fungus
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Issued Date
2011-06-01
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ISSN
13600478
09583157
09583157
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2-s2.0-79956118183
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Mahidol University
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SCOPUS
Bibliographic Citation
Biocontrol Science and Technology. Vol.21, No.6 (2011), 677-686
Suggested Citation
Duriya Chantasingh, Supattra Kitikhun, Lily Eurwilaichitr, Tanaporn Uengwetwanit, Kusol Pootanakit Functional expression in Beauveria bassiana of a chitinase gene from Ophiocordyceps unilateralis, an ant-pathogenic fungus. Biocontrol Science and Technology. Vol.21, No.6 (2011), 677-686. doi:10.1080/09583157.2011.570430 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11310
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Title
Functional expression in Beauveria bassiana of a chitinase gene from Ophiocordyceps unilateralis, an ant-pathogenic fungus
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Abstract
A gene encoding chitinase was cloned from Ophiocordyceps unilateralis, a Formamidae-specific fungus, collected from Sirindhorn Peat Swamp Forest, Thailand. The O. unilateralis chitinase (OuChi) full-length gene (1311 bp) encodes 436 amino acids with the first 20 amino acids as a putative signal peptide. The gene showed highest identity (78%) to Isaria farinose endochitinase. To investigate if cross-species chitinase expression also enhances fungal toxicity, the mature OuChi gene was subcloned into an Agrobacterium binary vector pPZP-bar and then transformed into Beauveria bassiana strain BCC2659. Chitinase activity was detected using 4-methylumbelliferyl-β-D-N,N′-diacetylchitobioside. The fungal transformant expressing O. unilateralis chitinase showed higher toxicity against Spodoptera exigua. These results support the hypothesis that chitinolytic enzymes are one of several 'virulence' factors produced by entomopathogenic fungi during host encounter. © 2011 Taylor & Francis.