Therapeutic potential of red bean (phaseolus vulgaris) peptides: anticancer, antihypertension, and antioxidant activities
Issued Date
2025-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-105021042888
Pubmed ID
41198802
Journal Title
Scientific Reports
Volume
15
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.15 No.1 (2025)
Suggested Citation
Roytrakul S., Jaresitthikunchai J., Charoenlappanit S., Thaisakun S., Phaonakrop N., Kittisenachai S., Supaibulwatana K. Therapeutic potential of red bean (phaseolus vulgaris) peptides: anticancer, antihypertension, and antioxidant activities. Scientific Reports Vol.15 No.1 (2025). doi:10.1038/s41598-025-22843-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113141
Title
Therapeutic potential of red bean (phaseolus vulgaris) peptides: anticancer, antihypertension, and antioxidant activities
Corresponding Author(s)
Other Contributor(s)
Abstract
Red beans (Phaseolus vulgaris) are gaining recognition for their potential in functional foods and nutraceuticals. This study isolated bioactive peptides under 3 kDa from red bean protein hydrolysate, generated by pepsin digestion and purified using sequential cation exchange, anion exchange, and reverse-phase chromatography. The resulting hydrolysate exhibited anticancer activity. Following chromatographic fractionation and LC-MS sequencing, eight candidate peptides were synthesized and comparatively assessed for bioactivity. Remarkably, peptide RB-8 (LIIPATSTKFL) demonstrated significant anticancer activity against colorectal (CaCO2) and cervical (SiHa) cancer cells through distinct mechanistic pathways. Furthermore, RB-7 (RGSKQRQKRQW) showed potent antihypertensive and DPPH radical scavenging activities, RB-6 (RRLRILL) displayed the strongest ABTS radical scavenging activity, and RB-1 (NLRKLKRL) exhibited the highest FRAP. These findings highlight the potential of red bean-derived peptides as natural agents for managing non-communicable diseases (NCDs) and their applicability in the food industry.