Probiotic-enriched Maoberry juice powder: Effects of carrier dextrose equivalent on physicochemical characteristics and gut microbiome
Issued Date
2025-12-01
Resource Type
eISSN
27725022
Scopus ID
2-s2.0-105022306873
Journal Title
Applied Food Research
Volume
5
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Applied Food Research Vol.5 No.2 (2025)
Suggested Citation
Prangthip P., Chaikham P. Probiotic-enriched Maoberry juice powder: Effects of carrier dextrose equivalent on physicochemical characteristics and gut microbiome. Applied Food Research Vol.5 No.2 (2025). doi:10.1016/j.afres.2025.101514 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113261
Title
Probiotic-enriched Maoberry juice powder: Effects of carrier dextrose equivalent on physicochemical characteristics and gut microbiome
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Corresponding Author(s)
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Abstract
The development of functional foods incorporating probiotics with bioactive fruit components has emerged as a promising approach for enhancing gut health and overall wellness. This study evaluated maltodextrin dextrose equivalent (DE) effects on spray-dried Maoberry ( Antidesma bunius ) juice enriched with Lacticaseibacillus casei 01 using DE10, DE15, and DE20 carriers. Among the formulations tested, DE10 provided superior protection, yielding the highest viable count (2.68 ± 0.73 × 10<sup>12</sup> CFU/ g) and phenolic retention, with gallic acid at 105.64±2.67 mg/100 g versus 85.37±5.93 mg/100 g for DE20. DE10 powders exhibited lower hygroscopicity (25.64 ± 1.53 vs 33.16 ± 2.18 g/100 g) and higher glass transition temperature (65.42 ± 1.68 °C vs 55.64 ± 3.17 °C), promoting storage stability. In vitro digestion demonstrated enhanced probiotic survival for DE10 (3.71 log reduction) compared to free cells (6.47 log reduction). In vivo administration resulted in a 70.91 % increase in total short-chain fatty acids (from 0.55 ± 0.04 to 0.94 ± 0.08 g/L) and 25.93 % reduction in total biogenic amines. Microbiological analysis showed increased beneficial bifidobacteria and lactobacilli with reduced pathogenic coliforms and clostridia. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiling revealed improved microbial diversity following supplementation. This DE10-based synbiotic powder represents a viable functional food ingredient with demonstrated prebiotic-probiotic synergy for gut health applications and therapeutic interventions.