Laboratory diagnosis of melioidosis
2
Issued Date
2025-12-01
Resource Type
eISSN
19352735
Scopus ID
2-s2.0-105023911806
Pubmed ID
41343561
Journal Title
Plos Neglected Tropical Diseases
Volume
19
Issue
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
Plos Neglected Tropical Diseases Vol.19 No.12 (2025) , e0013761
Suggested Citation
Gassiep I., Chewapreecha C., Chantratita N., Oakley T., Mukhopadhyay C., Lal P.B., AuCoin D., Chowdhury F.R., Meumann E.M., Currie B.J., Dance D.A.B., Wuthiekanun V., Norton R. Laboratory diagnosis of melioidosis. Plos Neglected Tropical Diseases Vol.19 No.12 (2025) , e0013761. doi:10.1371/journal.pntd.0013761 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113497
Title
Laboratory diagnosis of melioidosis
Author's Affiliation
The University of Queensland
London School of Hygiene & Tropical Medicine
Nuffield Department of Medicine
Royal Brisbane and Women's Hospital
Kasturba Medical College, Manipal
Faculty of Tropical Medicine, Mahidol University
Menzies School of Health Research
University of Nevada, Reno School of Medicine
UQ Centre for Clinical Research
Mahidol Oxford Tropical Medicine Research Unit
Royal Darwin Hospital
Mahosot Hospital, Lao
Mater Hospital Brisbane
Bangladesh Medical University
Territory Pathology
Parasites and Microbes Programme
London School of Hygiene & Tropical Medicine
Nuffield Department of Medicine
Royal Brisbane and Women's Hospital
Kasturba Medical College, Manipal
Faculty of Tropical Medicine, Mahidol University
Menzies School of Health Research
University of Nevada, Reno School of Medicine
UQ Centre for Clinical Research
Mahidol Oxford Tropical Medicine Research Unit
Royal Darwin Hospital
Mahosot Hospital, Lao
Mater Hospital Brisbane
Bangladesh Medical University
Territory Pathology
Parasites and Microbes Programme
Corresponding Author(s)
Other Contributor(s)
Abstract
Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is an infectious disease with high rates of morbidity and mortality, which primarily affects low- and middle-income countries in South and Southeast Asia and Australia. The clinical manifestations of this disease are nonspecific and, therefore, rapid laboratory diagnosis is especially critical as appropriate management requires specific antimicrobials. This article aims to provide an overview of the current diagnostic methodologies, emerging technologies, susceptibility testing, and future perspectives for laboratory diagnosis of melioidosis. By examining conventional culture methods, mass spectrometry, antimicrobial susceptibility testing, antigen detection, molecular diagnostics, and serological assays, this article highlights the current challenges in accurately and cost-effectively diagnosing melioidosis in diverse clinical and resource-limited settings. A detailed analysis of current and future diagnostic methodologies will offer valuable insights for clinicians, researchers, and public health professionals. This review aims to influence clinical and laboratory guidelines for diagnosing melioidosis and future research directions.