Molecular and functional analysis of Glucose-6-phosphate dehydrogenase variants
Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xi, 76 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Tropical Medicine)--Mahidol University, 2021
Suggested Citation
Aun Praoparotai Molecular and functional analysis of Glucose-6-phosphate dehydrogenase variants. Thesis (M.Sc. (Tropical Medicine)--Mahidol University, 2021. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113805
Title
Molecular and functional analysis of Glucose-6-phosphate dehydrogenase variants
Author(s)
Advisor(s)
Abstract
Deficiency of glucose-6-phosphate dehydrogenase (G6PD) is a hereditary genetic defect which is one of the most prevalent polymorphisms and enzymopathies in humans, affecting approximately 500 million people worldwide. Antimalarial medications, such as primaquine and tafenoquine, have been linked to the hemolysis in the G6PD-deficient individuals. As a result, hemolytic toxicity caused by G6PD deficiency has a significant impact on the malaria treatment, particularly in the malaria-endemic areas. In addition, the risk of hemolytic anemia varies widely among the G6PD-deficient individuals. In order to understand the molecular mechanisms underlying the clinical features of G6PD deficiency, the biochemical properties and structural stability of eleven G6PD variants which included three single, seven double, and one triple variants were investigated in this study. All G6PD variants were constructed, expressed, purified, and biochemically characterized. G6PD A, a neutral variant, shared the similar biochemical properties and structural staiblity to the native enzyme. Among other G6PD variants studied here, the structural instability largely contributed to the enzyme deficiency and, as a consequence, to clinical manifestations.The cumulative effects between two and three mutations, sharing G6PD A and causing the G6PD deficiency, were observed in the G6PD variants with double and triple mutations.
Description
Tropical Medicine (Mahidol University, 2021)
Degree Name
Master of Science
Degree Level
Masters
Degree Department
Faculty of Tropical Medicine
Degree Discipline
Tropical Medicine
Degree Grantor(s)
Mahidol University