Complete genomes of Rickettsia typhi reveal a clonal population
Issued Date
2025-12-01
Resource Type
eISSN
19352735
Scopus ID
2-s2.0-105027171652
Pubmed ID
41460881
Journal Title
Plos Neglected Tropical Diseases
Volume
19
Issue
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
Plos Neglected Tropical Diseases Vol.19 No.12 (2025) , e0013828
Suggested Citation
Keeratipusana C., Phuklia W., Phommadeechack V., Thaipadungpanit J., Chansamouth V., Phommasone K., Rattanavong S., Moore C.E., Robinson M.T., Richards A.L., Newton P.N., Batty E.M. Complete genomes of Rickettsia typhi reveal a clonal population. Plos Neglected Tropical Diseases Vol.19 No.12 (2025) , e0013828. doi:10.1371/journal.pntd.0013828 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/114045
Title
Complete genomes of Rickettsia typhi reveal a clonal population
Corresponding Author(s)
Other Contributor(s)
Abstract
Murine typhus, caused by infection with Rickettsia typhi, is a neglected disease contributing to infectious disease burden in south- and southeast Asia. Despite its importance, we have minimal knowledge of the genomics of R. typhi, with only four complete genomes being sequenced prior to this work. We sequenced a further 25 genomes including historical strains collected before 1976 from both human and rat hosts, and recent genomes isolated from patients at a single hospital in Laos. Whole genome SNP analysis reveals extremely low levels of genetic diversity across the 29 genomes, with overall nucleotide diversity (π) of 1.27e-05 and evidence of purifying selection, and a minimal pan-genome. Phylogenetic analysis shows clustering of the genome by historic or modern origin, with the exception of one modern strain which is most closely related to historic strains from Thailand, and no clustering by host origin. The highly conserved genome of R. typhi suggests strong constraints on genome evolution in this obligate intracellular parasite, and has implications for the design of future murine typhus diagnostic tools and vaccines.