Phage-based diagnostic assay for melioidosis using multiplex PCR targeting Burkholderia phage terminase genes
Issued Date
2026-03-20
Resource Type
eISSN
25890042
Scopus ID
2-s2.0-105029697459
Journal Title
Iscience
Volume
29
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
Iscience Vol.29 No.3 (2026)
Suggested Citation
Withatanung P., Vanaporn M., Chantratita N., Chareonsudjai S., Muangsombut V., Janesomboon S., Asawalertsaeng T., Clokie M.R.J., Galyov E.E., Korbsrisate S. Phage-based diagnostic assay for melioidosis using multiplex PCR targeting Burkholderia phage terminase genes. Iscience Vol.29 No.3 (2026). doi:10.1016/j.isci.2026.114831 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/115109
Title
Phage-based diagnostic assay for melioidosis using multiplex PCR targeting Burkholderia phage terminase genes
Corresponding Author(s)
Other Contributor(s)
Abstract
Conventional diagnostic approaches involve identifying the bacterial pathogen from patient samples, which can delay results and miss low-bacteremia cases. Herein, we demonstrate that Burkholderia phages circulating in patient blood can serve as a powerful diagnostic biomarker. Building on this, a multiplex PCR targeting the Burkholderia pseudomallei phage terminase gene was developed to improve melioidosis diagnosis. The assay provided 97.2% sensitivity and 100% specificity, with results obtainable within 6 h. The strength of this PCR assay is the amplification of four different multicopy Burkholderia phage terminase genes from serum samples, resulting in increased sensitivity under low-bacteremia conditions. To our knowledge, this study reports a phage-based PCR assay that detects phage DNA directly from melioidosis patients’ blood, representing a shift from molecular pathogen-based to phage-based diagnostics. This approach not only improves sensitivity but also opens avenues for integrating phage biology into the diagnostic landscape of infectious diseases.