Publication: Optimization of culture conditions for mycoepoxydiene production by Phomopsis sp. Hant25
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Issued Date
2011-06-01
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ISSN
14765535
13675435
13675435
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2-s2.0-80051667122
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Industrial Microbiology and Biotechnology. Vol.38, No.6 (2011), 679-685
Suggested Citation
Narukjaporn Thammajaruk, Nongluksna Sriubolmas, Duangnate Israngkul, Vithaya Meevootisom, Suthep Wiyakrutta Optimization of culture conditions for mycoepoxydiene production by Phomopsis sp. Hant25. Journal of Industrial Microbiology and Biotechnology. Vol.38, No.6 (2011), 679-685. doi:10.1007/s10295-010-0813-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11538
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Title
Optimization of culture conditions for mycoepoxydiene production by Phomopsis sp. Hant25
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Abstract
Culture media and fermentation conditions for cultivation of an endophytic fungus Phomopsis sp. Hant25 were investigated in order to improve the yield of mycoepoxydiene, a novel fungal metabolite having potent cytotoxic activity against many cancer cell lines. Mycoepoxydiene accumulated in the culture broth during the stationary phase of fungal growth. Modified M1D medium was superior to malt Czapek, and Czapek yeast autolysate broths in supporting mycoepoxydiene production. Pellet growth was the morphological form that favored biosynthesis of mycoepoxydiene. This could be achieved by incubating the culture statically for 6 days before shaking at 120 rpm. Incorporation of a cellulose paper disc into the culture flask promoted fungal growth at the liquid surface, which accelerated mycoepoxydiene production and maximized the final yield to a level of 354 mg l -1 , though fungal attachment to the solid support was not required. Since the peak concentration of mycoepoxydiene in the culture broth was followed by a steeply declining phase, the harvest time had to be precisely determined for maximum product yield. Understanding the factor(s) involved in rapid degradation of mycoepoxydiene could lead to improved final yields. © Society for Industrial Microbiology 2010.