UHPLC Analysis of Phenolic Compounds from Phanera strychnifolia Leaf Extracts, and Their Alpha-glucosidase and Alpha-Amylase Inhibitory Activities Supported by Molecular Docking
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Issued Date
2026-08-01
Resource Type
eISSN
27740226
Scopus ID
2-s2.0-105037821229
Journal Title
Trends in Sciences
Volume
23
Issue
8
Rights Holder(s)
SCOPUS
Bibliographic Citation
Trends in Sciences Vol.23 No.8 (2026)
Suggested Citation
Kongkiatpaiboon S., Tayana N., Seaho B., Schinnerl J., Duangdee N., Inthakusol W., Mudjupa C., Chewchinda S. UHPLC Analysis of Phenolic Compounds from Phanera strychnifolia Leaf Extracts, and Their Alpha-glucosidase and Alpha-Amylase Inhibitory Activities Supported by Molecular Docking. Trends in Sciences Vol.23 No.8 (2026). doi:10.48048/tis.2026.13308 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116654
Title
UHPLC Analysis of Phenolic Compounds from Phanera strychnifolia Leaf Extracts, and Their Alpha-glucosidase and Alpha-Amylase Inhibitory Activities Supported by Molecular Docking
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Abstract
Phanera strychnifolia is a traditional medicinal plant showing remarkable antihyperglycemic activity. To develop as a phytopharmaceutical product for Diabetes, an in-depth investigation of the α-glucosidase and α-amylase inhibitory activities of the main constituents was conducted. The developed UHPLC method was validated for qualitative and quantitative analyses of the major constituents gallic acid (1), trilobatin (2), yanangdaengin (3), and phloretin (4). The performed stress tests provide information about storage conditions and about the stabilities of these compounds under various conditions. The optimized extraction with ethanol/water (1:1, v/v) gave the highest yield of gallic acid (1), and the dihydrochalcones trilobatin (2), yanangdaengin (3), and phloretin (4). Yanangdaengin (3) exhibited potent α-glucosidase inhibitory activity (IC<inf>50</inf> of 10.42 µM), which was about 30 times higher active than the commercial drug acarbose (IC<inf>50</inf> of 329.09 µM). This result was also confirmed by computerized molecular docking. Our work confirms the suitability of hydrophilic leaf extracts from P. strychnifolia as a potential herbal medicine for the treatment of postprandial hyperglycemia and provides valuable information for the phytopharmaceutical development of this plant.