Co-immunization with recombinant S5196–272 and S6200–317 proteins for enhanced protective antibody response against Tilapia lake virus in Nile tilapia, Oreochromis niloticus (Linnaeus, 1758)
3
Issued Date
2025-09-01
Resource Type
ISSN
24681458
eISSN
2452316X
Scopus ID
2-s2.0-105039470592
Journal Title
Agriculture and Natural Resources
Volume
59
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
Agriculture and Natural Resources Vol.59 No.5 (2025)
Suggested Citation
Plysup B., Lueangyangyuen A., Khrisanapant P., Senapin S., Rattanarojpong T., Somsoros W., Khunrae P., Sangsuriya P. Co-immunization with recombinant S5196–272 and S6200–317 proteins for enhanced protective antibody response against Tilapia lake virus in Nile tilapia, Oreochromis niloticus (Linnaeus, 1758). Agriculture and Natural Resources Vol.59 No.5 (2025). doi:10.34044/j.anres.2025.59.5.14 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116970
Title
Co-immunization with recombinant S5196–272 and S6200–317 proteins for enhanced protective antibody response against Tilapia lake virus in Nile tilapia, Oreochromis niloticus (Linnaeus, 1758)
Corresponding Author(s)
Other Contributor(s)
Abstract
Importance of the work: Co-immunization with recombinant S5<inf>196–272</inf> and S6<inf>200–317</inf> proteins enhances protective immunity and provides insights for future TiLV vaccine development. Objectives: To evaluate the vaccine potential of combined S5<inf>196–272</inf> and S6<inf>200–317</inf> compared to individual immunization. Materials and Methods: A sample of Nile tilapia was divided into three main groups: immunized with S5<inf>196–272</inf> and S6<inf>200–317</inf> individually, or co-immunized. Antibody responses were measured weekly using enzyme-linked immunosorbent assay, with virus neutralization being assessed using a methylthiazolyldiphenyl-tetrazolium bromide (MTT) cell viability assay. A viral challenge test was conducted to determine the relative percentage of survival (RPS). Results: Co-immunization of the fish with S5<inf>196–272</inf> and S6<inf>200–317</inf> resulted in a synergistic effect, leading to the higher production of S6<inf>200–317</inf>-specific antibodies than for immunization with S6<inf>200–317</inf> alone. A significant increase in serum antibody levels was observed from 7 d, 21 d, 28 d and 35 d post-co-immunization. In contrast, S5<inf>196–272</inf>-specific antibodies were generated at consistently high levels following both individual and co-immunization. The MTT cell viability assay findings demonstrated that antibodies from the co-immunization group had the highest virus-neutralizing effect (87.22% viability). Furthermore, the viral challenge assay revealed that the co-immunization group had the highest RPS (57.14%), whereas individual immunization provided no protection effect against TiLV infection. Main finding: Co-immunization with S5<inf>196–272</inf> and S6<inf>200–317</inf> induced a synergistic antibody response and provided effective protection against TiLV in Nile tilapia.