Plant-based production and characterization of monoclonal antibodies targeting extracellular enveloped proteins of the Mpox Virus
1
Issued Date
2026-09-01
Resource Type
eISSN
2215017X
Scopus ID
2-s2.0-105039895418
Journal Title
Biotechnology Reports
Volume
51
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biotechnology Reports Vol.51 (2026)
Suggested Citation
Jirananon K., Shanmugaraj B., Manopwisedjaroen S., Thitithanyanont A., Teengam P., Phoolcharoen W. Plant-based production and characterization of monoclonal antibodies targeting extracellular enveloped proteins of the Mpox Virus. Biotechnology Reports Vol.51 (2026). doi:10.1016/j.btre.2026.e00962 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/117026
Title
Plant-based production and characterization of monoclonal antibodies targeting extracellular enveloped proteins of the Mpox Virus
Corresponding Author(s)
Other Contributor(s)
Abstract
The recent mpox outbreaks in non-endemic countries highlight the urgent need for improved therapeutics and diagnostics. In this study, monoclonal antibodies (mAbs) targeting the mpox enveloped virion antigens A35 and B6 were transiently expressed in Nicotiana benthamiana using a geminiviral vector system. Following agroinfiltration, anti-A35 and anti-B6 mAbs accumulated to 27 µg/g fresh weight at 3 days and 260 µg/g at 7 days post-infiltration, respectively. SDS-PAGE and Western blot analyses confirmed the assembly of antibodies, and the purified antibodies bound to mpox-infected Vero cells. The neutralization assays demonstrated moderate reductions in viral infection under the tested conditions. Additionally, an electrochemical immunosensor demonstrated the ability of plant-produced antibodies to detect mpox virus through antigen–antibody binding induced current changes. These results support that the plant-based systems as rapid platforms for producing mpox-specific antibodies for diagnostics and antiviral research.