Bone regenerative efficacy of binder-jet fabricated hydroxyapatite granules with and without biomimetic octacalcium phosphate-coated modification in a rat critical-sized calvarial defect model
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Issued Date
2026-01-01
Resource Type
ISSN
20563418
eISSN
20563426
Scopus ID
2-s2.0-105040006877
Journal Title
Regenerative Biomaterials
Volume
13
Rights Holder(s)
SCOPUS
Bibliographic Citation
Regenerative Biomaterials Vol.13 (2026)
Suggested Citation
Suwanprateeb J., Thammarakcharoen F., Suvannapruk W., Luangwattanawilai T., Rattanapinyopituk K., Sriwatananukulkit O., Hemstapat R. Bone regenerative efficacy of binder-jet fabricated hydroxyapatite granules with and without biomimetic octacalcium phosphate-coated modification in a rat critical-sized calvarial defect model. Regenerative Biomaterials Vol.13 (2026). doi:10.1093/rb/rbag076 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/117057
Title
Bone regenerative efficacy of binder-jet fabricated hydroxyapatite granules with and without biomimetic octacalcium phosphate-coated modification in a rat critical-sized calvarial defect model
Corresponding Author(s)
Other Contributor(s)
Abstract
Bone substitutes fabricated via additive manufacturing offer promising solutions for bone grafting. This study investigated the bone regeneration potential of binder-jet fabricated hydroxyapatite (HA) and biomimetic octacalcium phosphate (OCP)-coated binder-jet fabricated HA (HA-B) granules in a rat critical-sized calvarial defect model. Bone graft in the form of granules was produced via binder jet 3D printing combined with a phase transformation process and implanted into 5 mm defects for 4 and 12 weeks, with empty defects as controls. New bone formation, mechanical integration and cellular responses were assessed using micro-computed tomography, histology, histomorphometry, push-out testing and immunohistochemistry. Both HA and HA-B supported bone formation without local or systemic adverse effects. Osteoblast, osteoclast and osteocyte counts indicated active remodeling in bone graft implanted groups. Micro-CT showed significantly higher bone volume in HA-B than HA and Empty at 12 weeks, while histomorphometry showed differences only between HA-B and Empty. HA-B also exhibited the highest push-out force, indicating superior mechanical integration. These results demonstrate that binder-jet fabricated HA and OCP-coated HA granules are safe, osteoconductive, and effective for bone repair, with OCP coating further enhancing bone formation and material-host integration.