Publication: Investigation of HC04 human hepatocyte cells interaction with fibronectin and gelatin plated on paper for cell based sensor application
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Issued Date
2011-12-01
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2-s2.0-84860483312
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Mahidol University
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SCOPUS
Bibliographic Citation
BMEiCON-2011 - 4th Biomedical Engineering International Conference. (2011), 93-95
Suggested Citation
U. Waiwijit, A. Wisitsoraat, A. Tuantranont, B. Oonkhanond, W. Kandhavivorn Investigation of HC04 human hepatocyte cells interaction with fibronectin and gelatin plated on paper for cell based sensor application. BMEiCON-2011 - 4th Biomedical Engineering International Conference. (2011), 93-95. doi:10.1109/BMEiCon.2012.6172026 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11872
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Title
Investigation of HC04 human hepatocyte cells interaction with fibronectin and gelatin plated on paper for cell based sensor application
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Abstract
Recently, paper based biosensors have been studied in the fields of biology, chemistry, pharmacology and medicine. The main advantages of paper based biosensors are low cost, biocompatibility, portability and environmental compatibility. The process of cell immobilization on paper is an important step for the development of disposable paper based biosensors with a whole-cell electrochemical electrode. The major problem of this process is poor cell viability on paper. In this work, we study the viability of HC04 human hepatocyte cells immobilized on filter papers coated with gelatin and fibronectin. HC04 cells were cultured on uncoated and coated papers in a 5% CO 2 incubator for 6 and 24 hrs. Cell viability was then detected by tryphan blue and propidium iodide staining. The results show that % cell viability on gelatin and fibronectin coated paper are more than that on uncoated one. Therefore, the developed gelatin and fibronectin coated paper is promising for cell based biosensor applications. © 2011 IEEE.