Publication: Competitive suppression between chikungunya and dengue virus in Aedes albopictus C6/36 cell line
Issued Date
2011-12-01
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ISSN
01251562
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2-s2.0-84857766149
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.42, No.6 (2011), 1388-1394
Suggested Citation
Rutcharin Potiwat, Narumon Komalamisra, Usavadee Thavara, Apiwat Tawatsin, Padet Siriyasatien Competitive suppression between chikungunya and dengue virus in Aedes albopictus C6/36 cell line. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.42, No.6 (2011), 1388-1394. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/12189
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Title
Competitive suppression between chikungunya and dengue virus in Aedes albopictus C6/36 cell line
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Abstract
Aedes albopictus C6/36 cell line was used to evaluate dengue virus serotype-3 (DENV-3) and chikungunya virus (CHIKV) co-infection. Virus infection was determined using a one-step duplex reverse transcriptase polymerase chain reaction (D-RT-PCR). D-RT-PCR was positive for both viruses when equal multiplicity of infection (MOI) was utilized. Co-infection with different titers between a higher CHIKV titer (MOI = 1.0) than DENV-3 titer (MOI = 0.1) showed similar results with that of equal titer. However, co-infection with a lower CHIKV titer (MOI = 0.1) than DENV-3 titer (MOI = 1.0) revealed positive results for only dengue virus infection, suggesting DENV competitive suppression of CHIKV. This competitive suppression occurred in mixed-infection samples but not in individually double infection (super infection) samples which produced both dengue and chikungunya virus progenies. Both virus replications depend on the amount of virus titer rather than serial infection. These findings have provided information regarding pathogen-pathogen interaction in host cell, which could be used to predict outbreaks, and to develop virus detection and vector control.