Publication: Relation between Low Erythrocyte Acetylcholinesterase Activity and Membrane Lipids in Paroxysmal Noctural Haemoglobinuria
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Issued Date
1979-01-01
Resource Type
ISSN
13652141
00071048
00071048
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2-s2.0-0018427676
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Mahidol University
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SCOPUS
Bibliographic Citation
British Journal of Haematology. Vol.41, No.3 (1979), 383-391
Suggested Citation
Junjaree Siriwittayakorn, Yongyuth Yuthavong Relation between Low Erythrocyte Acetylcholinesterase Activity and Membrane Lipids in Paroxysmal Noctural Haemoglobinuria. British Journal of Haematology. Vol.41, No.3 (1979), 383-391. doi:10.1111/j.1365-2141.1979.tb05871.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13253
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Title
Relation between Low Erythrocyte Acetylcholinesterase Activity and Membrane Lipids in Paroxysmal Noctural Haemoglobinuria
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Abstract
Summary. Acetylcholinesterase of intact erythrocytes, their ghosts and salt soluble extracts obtained from patients with paroxysmal nocturnal haemoglobinuria (PNH) does not differ from normal with respect to K m values for acetylthiocholine, and K i values for phenyltrimethylammonium iodide. However, the enzyme from PNH sources has lower V max values than normal, has different thermal stability from normal, has less distinctive transition temperature in the Arrhenius plots, and is less subject to inhibition by stearic acid. These results and that from comparison of activation of deoxycholate‐extracted enzyme by lipids from normal erythrocytes suggest that the low acetylcholinesterase activity in PNH erythrocytes is due, at least in part, to alteration in the lipid environment of the enzyme. Copyright © 1979, Wiley Blackwell. All rights reserved