Publication: Birth of Kittens After the Transfer of Frozen-Thawed Embryos Produced by Intracytoplasmic Sperm Injection with Spermatozoa Collected from Cryopreserved Testicular Tissue
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Issued Date
2012-12-01
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ISSN
14390531
09366768
09366768
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2-s2.0-84871703974
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Mahidol University
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SCOPUS
Bibliographic Citation
Reproduction in Domestic Animals. Vol.47, No.SUPPL. 6 (2012), 305-308
Suggested Citation
T. Tharasanit, S. Buarpung, S. Manee-In, C. Thongkittidilok, N. Tiptanavattana, P. Comizzoli, M. Techakumphu Birth of Kittens After the Transfer of Frozen-Thawed Embryos Produced by Intracytoplasmic Sperm Injection with Spermatozoa Collected from Cryopreserved Testicular Tissue. Reproduction in Domestic Animals. Vol.47, No.SUPPL. 6 (2012), 305-308. doi:10.1111/rda.12072 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13363
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Title
Birth of Kittens After the Transfer of Frozen-Thawed Embryos Produced by Intracytoplasmic Sperm Injection with Spermatozoa Collected from Cryopreserved Testicular Tissue
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Abstract
Contents: The aim of this study is to produce live kittens from oocytes fertilized by intracytoplasmic sperm injection (ICSI) with frozen/thawed testicular spermatozoa. Spermatozoa were collected from thawed testicular tissue and subsequently injected into in vitro matured cat oocytes. At 24 h post-ICSI, presumptive zygotes/cleaved embryos were treated with 10 μm forskolin for 24 h to reduce intracellular lipid content of embryos (delipidation). At 48 h after oocyte injection, cleaved embryos (2- to 8-cell stage) were frozen in 10% (v/v) ethylene glycol-based medium by a slow controlled rate method and stored in liquid nitrogen. To evaluate in vitro and in vivo developmental competence, frozen embryos were thawed and then cultured for 6 days (n = 155) or cultured for 2 h before transferred (n = 209) to hormonal (equine chorionic gonadotropin/hCG)-treated cat recipients. Cleavage frequency at day 2 after ICSI with frozen/thawed testicular spermatozoa was ~30%. The percentages of frozen/thawed embryos that developed to morula and blastocyst stage (on day 3 and day 6 of in vitro culture, respectively) were significantly lower than that of fresh ICSI embryos (22.6 vs 45.2% and 21.3 vs 38.7%, respectively; p < 0.05). However, no difference was found in the number of blastomeres between frozen/thawed (242.5 ± 43.1) and fresh (320.2 ± 28.1) blastocysts. Three of seven cat recipients were pregnant and one pregnant cat delivered two healthy kittens. This is the first report of the birth of kittens after the transfer of frozen-thawed embryos produced by ICSI with frozen/thawed testicular sperm. © 2012 Blackwell Verlag GmbH.