Publication:
Characterization and evaluation of microsatellite markers in a strain of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), with a genetic sexing character used in sterile insect population control

8

Issued Date

2012-01-01

Resource Type

Article

ISSN

18028829
12105759

DOI

10.14411/eje.2012.043

Other identifier(s)

2-s2.0-84865172387

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

European Journal of Entomology. Vol.109, No.3 (2012), 331-338

Suggested Citation

Siriwan Isasawin, Nidchaya Aketarawong, Sujinda Thanaphum Characterization and evaluation of microsatellite markers in a strain of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), with a genetic sexing character used in sterile insect population control. European Journal of Entomology. Vol.109, No.3 (2012), 331-338. doi:10.14411/eje.2012.043 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13508

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Title

Characterization and evaluation of microsatellite markers in a strain of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), with a genetic sexing character used in sterile insect population control

Author(s)

Siriwan Isasawin
 Nidchaya Aketarawong
 Sujinda Thanaphum

Other Contributor(s)

Mahidol University

Abstract

The oriental fruit fly, Bactrocera dorsalis, is a key economic insect pest reducing fruit yield and generating constraints in the international market. The application of the sterile insect technique (SIT) continues to reveal areas where new technologies can improve the effectiveness of fruit fly control. One such advancement concerns insect strains. In the present study, a mass-reared strain of the fly with a translocation-based genetic sexing character (Salaya1) based on a brown-white pupal colour dimorphism was genetically characterized using 11 microsatellite DNA markers. Subsequently, these markers were used to evaluate the maintenance of genetic variability in the strain under mass-rearing conditions. Mating competitiveness of this strain was also tested in field cages. Two of the newly characterized Y-pseudo-linked microsatellite markers were used for strain identification in field monitoring traps. The strain was also validated in a pilot integrated pest management (IPM) programme using male-only SIT in a fruit orchard. The programme resulted in the suppression of the fruit fly population.

Keyword(s)

Agricultural and Biological Sciences

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URI

https://repository.li.mahidol.ac.th/handle/123456789/13508

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Scopus 2011-2015