Publication: Role of vitelline envelope during fertilization in the black tiger shrimp, Penaeus monodon
Issued Date
2012-11-01
Resource Type
ISSN
16180372
00651281
00651281
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2-s2.0-84863450943
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Histochemica. Vol.114, No.7 (2012), 659-664
Suggested Citation
Piyada Ngernsoungnern, Apichart Ngernsoungnern, Yupaporn Chaiseha, Prapee Sretarugsa Role of vitelline envelope during fertilization in the black tiger shrimp, Penaeus monodon. Acta Histochemica. Vol.114, No.7 (2012), 659-664. doi:10.1016/j.acthis.2011.11.013 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13580
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Title
Role of vitelline envelope during fertilization in the black tiger shrimp, Penaeus monodon
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Abstract
Animal eggs possess investments through which sperm must penetrate. The aim of the present study was to investigate the role of the egg coating, the vitelline envelope, during sperm-egg interactions in the black tiger shrimp, . Penaeus monodon. The site(s) of primary binding between sperm and egg and the possible binding molecule(s) for sperm were identified. . In vitro adsorption of the vitelline envelope protein onto the sperm surface showed that primary binding occurred between the sperm anterior spike of acrosome intact sperm and the vitelline envelope. Results from streptavidin blotting revealed that the component of the vitelline envelope that interacts with the sperm integral membrane protein is a 370. kDa protein. In addition, it was shown that the vitelline envelope protein had no ability to induce acrosome reaction. These results suggest that the function of the vitelline envelope is as a primary binding site for sperm in shrimp, but not a sole trigger for the acrosome reaction. © 2011 Elsevier GmbH.