Publication: The variability in tissue proteomics
Issued Date
2012-08-01
Resource Type
ISSN
18628354
18628346
18628346
Other identifier(s)
2-s2.0-84865603091
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Mahidol University
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SCOPUS
Bibliographic Citation
Proteomics - Clinical Applications. Vol.6, No.7-8 (2012), 340-342
Suggested Citation
Visith Thongboonkerd The variability in tissue proteomics. Proteomics - Clinical Applications. Vol.6, No.7-8 (2012), 340-342. doi:10.1002/prca.201270035 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13647
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Title
The variability in tissue proteomics
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Abstract
Variability is one of the most critical issues of concern in clinical proteomics. In this issue of Proteomics Clinical Applications, Yoshida et al. [Proteomics Clin. Appl. 2012, 6, 412-417] describe the effects of blood and number of washes on the human glomerular proteome isolated from the kidney by laser microdissection. The blood-derived proteins occupied almost 50% of all the identified proteins in the unwashed samples, whereas varying the number of washes (from 1-5) with PBS yielded only 43-80% of the proteins identified in each sample that were common in all samples. This urges caution to all proteomists to carefully consider sample preservation and preparation for tissue proteome analysis. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.