Publication: Identification and characterization of a collagen-binding protein, Cbm, in Streptococcus mutans
1
Issued Date
2012-08-01
Resource Type
ISSN
20411014
20411006
20411006
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2-s2.0-84863599161
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular Oral Microbiology. Vol.27, No.4 (2012), 308-323
Suggested Citation
R. Nomura, K. Nakano, S. Naka, H. Nemoto, K. Masuda, J. Lapirattanakul, S. Alaluusua, M. Matsumoto, S. Kawabata, T. Ooshima Identification and characterization of a collagen-binding protein, Cbm, in Streptococcus mutans. Molecular Oral Microbiology. Vol.27, No.4 (2012), 308-323. doi:10.1111/j.2041-1014.2012.00649.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/14079
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Title
Identification and characterization of a collagen-binding protein, Cbm, in Streptococcus mutans
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Abstract
Streptococcus mutans, a major pathogen of dental caries, is occasionally isolated from the blood of patients with infective endocarditis. Bacterial attachment of exposed collagen tissue in the impaired endothelium is an important step in the onset of infective endocarditis. In our previous studies, some S. mutans strains were shown to possess collagen-binding activities and most of them had an approximately 120-kDa cell-surface collagen-binding protein called Cnm. However, several strains without Cnm proteins show collagen-binding properties. In the present study, another collagen-binding protein, Cbm, was characterized and its coding gene cbm was sequenced in these strains. The amino acid alignment in the putative collagen-binding domain of Cbm was shown to have approximately 80% identity and 90% similarity to the comparable region of Cnm. Cbm-deficient isogenic mutant strains constructed by insertional inactivation of the cbm gene, lacked collagen-binding properties, which were recovered in the complemented mutant. Analyses of a large number of clinical isolates from Japan, Thailand and Finland revealed that cbm-positive strains were present in all of these countries and that cnm-positive and cbm-positive strains were detected in the oral cavity of approximately 10 and 2% of systemically healthy subjects, respectively. In addition, cnm-positive strains were predominantly identified in the serotype f group, whereas cbm-positive strains were frequently detected in serotype k. These results suggest that Cbm as well as Cnm are major cell surface proteins of S. mutans associated with binding to type I collagen and predominantly identified in serotype k strains. © 2012 John Wiley & Sons A/S.