Publication: Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
1
Issued Date
2012-10-01
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ISSN
18783503
00359203
00359203
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2-s2.0-84865990708
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Mahidol University
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SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.106, No.10 (2012), 632-635
Suggested Citation
Sontana Siritantikorn, Suthatip Jintaworn, Sansanee Noisakran, Yupin Suputtamongkol, Daniel H. Paris, Stuart D. Blacksell Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.106, No.10 (2012), 632-635. doi:10.1016/j.trstmh.2012.05.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/14259
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Title
Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
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Abstract
The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach. © 2012 Royal Society of Tropical Medicine and Hygiene.