Publication: Identification of differentially expressed proteins from Burkholderia pseudomallei isolated during primary and relapsing melioidosis
Issued Date
2012-04-01
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ISSN
1769714X
12864579
12864579
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2-s2.0-84857977822
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbes and Infection. Vol.14, No.4 (2012), 335-340
Suggested Citation
Billie Velapatiño, Direk Limmathurotsakul, Sharon J. Peacock, David P. Speert Identification of differentially expressed proteins from Burkholderia pseudomallei isolated during primary and relapsing melioidosis. Microbes and Infection. Vol.14, No.4 (2012), 335-340. doi:10.1016/j.micinf.2011.11.011 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/14343
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Title
Identification of differentially expressed proteins from Burkholderia pseudomallei isolated during primary and relapsing melioidosis
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Abstract
Burkholderia pseudomallei causes septicemic melioidosis with a high rate of relapse, however microbial determinants of relapse are unknown. Proteins were analyzed from sequential B. pseudomallei isolates from primary and relapsing melioidosis. Analysis by isotope tagging for relative and absolute quantitation revealed that factors required for nitric oxide detoxification (HmpA) and necessary for anaerobic growth (ArcA, ArcC and ArcB) were highly expressed in the relapse isolate. Two-dimensional gel electrophoresis revealed up-regulation of a putative hemolysin-coregulated protein in the primary isolate, and flagellin and HSP20/alpha crystalline in the relapse isolate. These observations provide targets for further analysis of latency and virulence of melioidosis. © 2011 Institut Pasteur.