Publication: Real-time PCR: Benefits for detection of mild and asymptomatic giardia infections
Issued Date
2012-10-30
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ISSN
13494147
13488945
13488945
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2-s2.0-84867855680
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Mahidol University
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SCOPUS
Bibliographic Citation
Tropical Medicine and Health. Vol.40, No.2 (2012), 31-35
Suggested Citation
Rapeepun Prasertbun, Yaowalark Sukthana, Supaluk Popruk Real-time PCR: Benefits for detection of mild and asymptomatic giardia infections. Tropical Medicine and Health. Vol.40, No.2 (2012), 31-35. doi:10.2149/tmh.2012-08 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/14585
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Title
Real-time PCR: Benefits for detection of mild and asymptomatic giardia infections
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Abstract
The majority of Giardia infections are transmitted by the fecal-oral route and cause giardiasis. Children who live in crowded conditions or low socio-economic areas are the risk group for Giardia infection. Interestingly, most of them are asymptomatic or only mildly infected and can shed the Giardia cysts in the environment. Thus, the diagnosis of Giardia infection in asymptomatic or mild infection plays an important role in achieving control of Giardia duodenalis transmission. The objective of this study was to examine parasitic infections using microscopy and to develop a real-time PCR method for detection of Giardia infection in the stool samples of children living on the Thai-Myanmar border. Both species-specific primers and fluorescent labeled G. duodenalis probe were designed using small-subunit ribosomal RNA (ssrRNA). The results showed that 10 (7.69%) and 40 (30.77%) of 130 stool samples were positive for G. duodenalis by microscopy and real-time PCR respectively. Only 3 out of 9 liquid stools revealed G. duodenalis positive using microscopy, but all of them were G. duodenalis-positive using real-time PCR. The detection limit of real-time PCR for G. duodenalis was 0.1 pg/25 μl reaction. It can detect both mild and asymptomatic Giardia infections in children living on the Thai-Myanmar border. © 2012 by The Japanese Society of Tropical Medicine.