Publication: Molecular analyses of TEM genes and their corresponding penicillinase-producing Neisseria gonorrhoeae isolates in Bangkok, Thailand
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Issued Date
2012-02-01
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ISSN
10986596
00664804
00664804
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2-s2.0-84856068457
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Mahidol University
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SCOPUS
Bibliographic Citation
Antimicrobial Agents and Chemotherapy. Vol.56, No.2 (2012), 916-920
Suggested Citation
Shu Ichi Nakayama, Chanwit Tribuddharat, Sasiprapa Prombhul, Ken Shimuta, Somporn Srifuengfung, Magnus Unemo, Makoto Ohnishi Molecular analyses of TEM genes and their corresponding penicillinase-producing Neisseria gonorrhoeae isolates in Bangkok, Thailand. Antimicrobial Agents and Chemotherapy. Vol.56, No.2 (2012), 916-920. doi:10.1128/AAC.05665-11 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15004
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Title
Molecular analyses of TEM genes and their corresponding penicillinase-producing Neisseria gonorrhoeae isolates in Bangkok, Thailand
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Abstract
Neisseria gonorrhoeae is a major public health problem globally, especially because the bacterium has developed resistance to most antimicrobials introduced for first-line treatment of gonorrhea. In the present study, 96 N. gonorrhoeae isolates with high-level resistance to penicillin from 121 clinical isolates in Thailand were examined to investigate changes related to their plasmid-mediated penicillin resistance and their molecular epidemiological relationships. A β-lactamase (TEM) gene variant, bla TEM-135 , that may be a precursor in the transitional stage of a traditional bla TEM-1 gene into an extended-spectrum β-lactamase (ESBL), possibly causing high resistance to all extended-spectrum cephalosporins in N. gonorrhoeae, was identified. Clonal analysis using multilocus sequence typing (MLST) and N. gonorrhoeae multiantigen sequence typing (NG-MAST) revealed the existence of a sexual network among patients from Japan and Thailand. Molecular analysis of the bla TEM-135 gene showed that the emergence of this allele might not be a rare genetic event and that the allele has evolved in different plasmid backgrounds, which results possibly indicate that it is selected due to antimicrobial pressure. The presence of the bla TEM-135 allele in the penicillinase-producing N. gonorrhoeae population may call for monitoring for the possible emergence of ESBL-producing N. gonorrhoeae in the future. This study identified a bla TEM variant (bla TEM-135 ) that is a possible intermediate precursor for an ESBL, which warrants international awareness. Copyright © 2012, American Society for Microbiology. All Rights Reserved.