Publication: Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis
Issued Date
1988-01-01
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ISSN
14698161
00311820
00311820
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2-s2.0-0023854491
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology. Vol.96, No.1 (1988), 119-128
Suggested Citation
S. Wongratanacheewin, R. Chawengkirttikul, D. Bunnag Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis. Parasitology. Vol.96, No.1 (1988), 119-128. doi:10.1017/S0031182000081701 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15485
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Title
Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis
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Abstract
Antigenicity of adult Opisthorchis viverrini, metacercariae and eggs was investigated by radio-immunoprecipitation and polyacrylamide gel electrophoresis using sera from patients with opisthorchiasis and from hamsters experimentally infected with O. viverrini meta-cercariae. 125 I-IabelIed aqueous somatic extract and in vitro metabolic products of adult worms, and the extracts of metacercariae and eggs were immunoprecipitated, electrophoresed and autoradiographed and major antigens were characterized. Although components with Mr of > 116, 89, 78 and 20 kD appeared to be specifically associated with the somatic extract of adult fluke, the 89 kD protein was the most prominent component found in the in vitro culture fluid of adult worms and the metacercarial extract. Hamsters, experimentally infected with O. viverrini, also showed the presence of antibody reactive with the 89 kD component within 15 days of infection. The potential of this antigen in immunodiagnosis is discussed. © 1988, Cambridge University Press. All rights reserved.