Publication: Cloning and expression of xylanase 10 from Cryptovalsa mangrovei (BCC7197) in Pichia pastoris
Issued Date
2005-10-01
Resource Type
ISSN
10292365
10425179
10425179
Other identifier(s)
2-s2.0-33645237350
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Mahidol University
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SCOPUS
Bibliographic Citation
DNA Sequence - Journal of DNA Sequencing and Mapping. Vol.16, No.5 (2005), 372-378
Suggested Citation
Katewadee Boonyapakron, Kusol Pootanakit, Duriya Chantasingh, Kanyawim Kirtikara, Lily Eurwilaichitr Cloning and expression of xylanase 10 from Cryptovalsa mangrovei (BCC7197) in Pichia pastoris. DNA Sequence - Journal of DNA Sequencing and Mapping. Vol.16, No.5 (2005), 372-378. doi:10.1080/10425170500186629 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/16288
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Title
Cloning and expression of xylanase 10 from Cryptovalsa mangrovei (BCC7197) in Pichia pastoris
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Abstract
Xylanases are one of the industrially valuable enzymes. Using RT-PCR and 5′- and 3′-RACE procedures, we have cloned a full-length xylanase encoding gene from a filamentous fungus, Cryptovalsa mangrovei (BCC7197) from Phuket, Thailand. The results showed that BCC7197 xylanase cDNA has an open reading frame of 978 bp encoding 325 amino acid residues. Further sequence analysis revealed that this xylanase gene is belonged to the glycosyl hydrolase family 10 and has ∼ 50-60% amino acid sequence similarity to other fungal xylanases. Furthermore, expression of BCC7197 xylanase in the Pichia pastoris was also performed. The results demonstrated that the active BCC7197 xylanase protein was successfully produced and secreted from P. pastoris. © 2005 Taylor & Francis.