Publication: Basic nuclear protein pattern and chromatin condensation in the male germ cells of a tropical abalone, Haliotis asinina
1
Issued Date
2005-02-01
Resource Type
ISSN
1040452X
Other identifier(s)
2-s2.0-11244312902
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Molecular Reproduction and Development. Vol.70, No.2 (2005), 211-221
Suggested Citation
Worawit Suphamungmee, Somjai Apisawetakan, Wattana Weerachatyanukul, Chaitip Wanichanon, Prapee Sretarugsa, Tanes Poomtong, Prasert Sobhon Basic nuclear protein pattern and chromatin condensation in the male germ cells of a tropical abalone, Haliotis asinina. Molecular Reproduction and Development. Vol.70, No.2 (2005), 211-221. doi:10.1002/mrd.20199 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/16378
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Basic nuclear protein pattern and chromatin condensation in the male germ cells of a tropical abalone, Haliotis asinina
Other Contributor(s)
Abstract
The basic nuclear proteins (BNPs) in spermatozoa of a tropical abalone, Haliotis asinina, were composed of a majority of protamine-like (PL) protein and a small amount of histones H1 and H4. Abalone H1 and PL proteins exhibited strong immunological cross reactivities among themselves as well as with chick H5 and calf thymus H1. Thus, all these proteins may belong to the same family. Immunolocalization by indirect immunofluorescence and immunoelectron microscopy indicated that H1 and H4 were present in all steps of the male germ cells, however, with decreasing amount in late stage cells, particularly spermatids and spermatozoa. On the other hand, PL was present in middle step cells (secondary spermatocytes) with increasing amount in spermatids and spermatozoa when the chromatin became tightly packed. Thus, PL may be involved in the condensation of chromatin in the spermatozoa of this species. © 2005 Wiley-Liss, Inc.