Publication: Attenuation of cytoadherence of Plasmodium falciparum to microvascular endothelium under flow by hemodilution
Issued Date
2005-06-01
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ISSN
00029637
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2-s2.0-23844478659
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.72, No.6 (2005), 660-665
Suggested Citation
Christine Flatt, Sheona Mitchell, Bryan Yipp, Sornchai Looareesuwan, May Ho Attenuation of cytoadherence of Plasmodium falciparum to microvascular endothelium under flow by hemodilution. American Journal of Tropical Medicine and Hygiene. Vol.72, No.6 (2005), 660-665. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/16578
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Title
Attenuation of cytoadherence of Plasmodium falciparum to microvascular endothelium under flow by hemodilution
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Abstract
The cytoadherence of Plasmodium falciparum-infected erythrocytes (IRBCs) to endothelium is mediated by adhesion molecules within the physical constraints of a viscous fluid containing mostly erythrocytes. The volume fraction of erythrocytes (hematocrit) and their physical properties, such as deformability, are important properties of blood that affect cell recruitment to the vascular wall. In the present study, we examined the effect of hematocrit on IRBC rolling and adhesion on human microvascular endothelial cells in a flow chamber system in vitro. We found hematocrit to be a major determinant of IRBC/endothelial cell interactions. There was a 5-fold and 12-fold increase in IRBC rolling and adhesion, respectively, when hematocrit increased from 10% to 30%, as a result of changes in shear rate. Similar effects were seen in the presence of less deformable erythrocytes, serum proteins, and on endothelium stimulated with tumor necrosis factor-α. The results indicate that hemorheologic variations are an important determinant of the degree of cytoadherence. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene.