Publication:
Simple nonradioactive DNA hybridization method for identification of sibling species of Anopheles dirus (Diptera: Culicidae) complex.

2

Issued Date

1995-01-01

Resource Type

Article

ISSN

00222585

DOI

10.1093/jmedent/32.2.107

Other identifier(s)

2-s2.0-0029268511

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of medical entomology. Vol.32, No.2 (1995), 107-111

Suggested Citation

M. Audtho, A. Tassanakajon, V. Boonsaeng, S. Tpiankijagum, S. Panyim Simple nonradioactive DNA hybridization method for identification of sibling species of Anopheles dirus (Diptera: Culicidae) complex.. Journal of medical entomology. Vol.32, No.2 (1995), 107-111. doi:10.1093/jmedent/32.2.107 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/17230

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Title

Simple nonradioactive DNA hybridization method for identification of sibling species of Anopheles dirus (Diptera: Culicidae) complex.

Author(s)

M. Audtho
 A. Tassanakajon
 V. Boonsaeng
 S. Tpiankijagum
 S. Panyim

Other Contributor(s)

Mahidol University

Abstract

A simple method was developed for species identification of mosquitoes in the Anopheles dirus complex found in Thailand using horseradish peroxidase-labeled DNA probes and a chemiluminescent detection system. Species-specific DNA probes for Anopheles dirus, species B, C, and D detected 1-5 ng of target DNA, a sensitivity that was comparable with the radioisotopic detection system. Identification of individual mosquitoes was performed by dot-blot analysis of crude mosquito DNA. The method allowed identification using third or fourth instars as well as the adult head, thorax, or abdomen. This technique successfully identified the sibling species of the A. dirus complex in field collections.

Keyword(s)

Agricultural and Biological Sciences
 Immunology and Microbiology
 Medicine
 Veterinary

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/17230

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Scopus 1991-2000