Publication: Alternative method for determination of pyrimethamine in plasma by high-performance liquid chromatography
Issued Date
1997-02-21
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ISSN
15726495
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2-s2.0-0031581910
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Chromatography B: Biomedical Applications. Vol.689, No.2 (1997), 433-437
Suggested Citation
K. Na-Bangchang, P. Tan-Ariya, R. Ubalee, B. Kamanikom, J. Karbwang Alternative method for determination of pyrimethamine in plasma by high-performance liquid chromatography. Journal of Chromatography B: Biomedical Applications. Vol.689, No.2 (1997), 433-437. doi:10.1016/S0378-4347(96)00327-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/17937
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Title
Alternative method for determination of pyrimethamine in plasma by high-performance liquid chromatography
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Abstract
A rapid, selective, sensitive and reproducible reversed-phase high-performance liquid chromatography (HPLC) procedure for the quantitative determination of pyrimethamine (PYR) in plasma is described. The procedure involved the two-step extraction of PYR and the internal standard, quinine (QN) with acetonitrile and dichloromethane at basic pH. Chromatographic separation consisted of the mobile phase (methanol-water containing 0.005 M octanesulfonic acid, 50:50, v/v) running through the column (Techopak-10 C18) at a flow-rate of 1.6 ml/min. Detection was at UV wavelength of 240 nm. The mean recoveries of PYR and QN at a concentration range of 50 and 500 ng/ml were 98.9 and 89%, and 94.7 and 96% for PYR and QN. The within-day coefficients of variation were 2.1-5.1% for PYR and 5.9% for QN. The day-to-day coefficients of variation were 2.1-4.1% for PYR and 5% for QN. The minimum detectable concentrations for PYR and QN in plasma were 3 and 10 ng/ml. The method was found to be suitable for use in clinical pharmacokinetic study.