Publication: Morphological alterations and apoptosis of endothelial cells induced by thalassemic serum in vitro
Issued Date
1997-12-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-0031309946
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, (1997), 149-154
Suggested Citation
Ratana Banjerdpongchai, Prapon Wilairat, Suthat Fucharoen, Ahnond Bunyaratvej Morphological alterations and apoptosis of endothelial cells induced by thalassemic serum in vitro. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, (1997), 149-154. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18054
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Title
Morphological alterations and apoptosis of endothelial cells induced by thalassemic serum in vitro
Abstract
Vascular complications such as lung thromboembolism and leg ulcer have been observed in thalassemic patients. Recently, our group has reported impaired proliferation of endothelial cells (ECs) after exposure to α- and β-thalassemic sera in a cultuie system This study was undertaken to detect apoptotic phenomena of ECs in the presence of α- and β-thalassemic serum. ECs from normal human umbilical cord vein were exposed to 30% thalassemlc serum in vitro and morphological changes were observed by using phase contrast, fluorescence and scanning electron microscopy. Such treated ECs presented morphological characteristics of apoptosis as shown by the appearance of compact cytosol. membrane blebbing, margination of nuclear matrix, condensed nuclei, and fragmented bodies. The majority of apoptotic cells was in the floating population. Similar morphological changes were also observed by treating ECs with hydrogen peroxide in the concentration range of 0.1 - 10 mM.