Publication: Hemoglobin typing by high performance liquid chromatography
2
Issued Date
1997-06-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-0031151484
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, No.2 (1997), 417-423
Suggested Citation
Thanusak Tatu, Prakaiwan Gategasem, Phongjan Hathirat Hemoglobin typing by high performance liquid chromatography. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, No.2 (1997), 417-423. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18122
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Title
Hemoglobin typing by high performance liquid chromatography
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Abstract
To evaluate the potential application of high performance liquid chromatography or HPLC in performing hemoglobin typing, comparison between this technique and routine methods was carried out. Blood specimens from Pediatric Hematology Unit, Research Center, Faculty of Medicine, Ramathibodi Hospital were examined by these methods. The level of Hb A2, Hb E and Hb F were compared. Hb A2 level determined by HPLC and electrophoresis was statistically significantly different but correlated well. Good correlation was found between Hb A2 level from HPLC compared with microcolumn chromatography although there was a statistical difference. No difference but very good correlation was found between the level of Hb E from HPLC compared with electrophoresis. Statistical difference was encountered when Hb F level determined by HPLC was compared to that determined by Betke alkaline denaturation test. However, good correlation was observed when the level of Hb F was greater than 2.0%. In conclusion, HPLC could be an alternative way of performing hemoglobin typing provided that Hb F is 10% or more, by calculating from the equation: Alk F = (0.83 × Hb F by HPLC) - 0.98.