Publication: Large scale culture technique for pure Plasmodium falciparum gametocytes
1
Issued Date
1997-03-01
Resource Type
ISSN
01251562
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2-s2.0-0031087759
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, No.1 (1997), 18-21
Suggested Citation
Porntip Petmitr, Ganokwan Pongvilairat, Prapon Wilairat Large scale culture technique for pure Plasmodium falciparum gametocytes. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, No.1 (1997), 18-21. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18150
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Title
Large scale culture technique for pure Plasmodium falciparum gametocytes
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Abstract
A large scale technique for pure gametocyte cultures of Plasmodium falciparum was established in culture flasks by using treated erythrocytes and RPMI medium supplemented with 15% human plasma instead of serum. Pure gametocyte cultures were successfully obtained following treatment with 5% sorbitol on day 8 and 9 of cultivation This method resulted in approximately 97% reduction of asexual parasites and provided pure gametocytes in culture. The highest numbers of gametocytes were obtained from cultures starting with 2% parasitemia and 2% erythrocyte suspension. On day 12 of cultivation, approximately 35 × 106 gametocytes per 100 ml of cell suspension could be harvested.