Publication: Biodegradation of crude oil by soil microorganisms in the tropic
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Issued Date
1998-11-11
Resource Type
ISSN
09239820
Other identifier(s)
2-s2.0-0031789071
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biodegradation. Vol.9, No.2 (1998), 83-90
Suggested Citation
Manee Palittapongarnpim, Prayad Pokethitiyook, Edward Suchart Upatham, Ladda Tangbanluekal Biodegradation of crude oil by soil microorganisms in the tropic. Biodegradation. Vol.9, No.2 (1998), 83-90. doi:10.1023/A:1008272303740 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18285
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Title
Biodegradation of crude oil by soil microorganisms in the tropic
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Abstract
Five microorganisms, three bacteria and two yeasts, capable of degrading Tapis light crude oil were isolated from oil-contaminated soil in Bangkok, Thailand. Soil enrichment culture was done by inoculating the soil in mineral salt medium with 0.5% v/v Tapis crude oil as the sole carbon source. Crude oil biodegradation was measured by gas chromatography method. Five strains of pure microorganisms with petroleum degrading ability were isolated: three were bacteria and the other two were yeasts. Candida tropicalis strains 7Y and 15Y were identified as efficient oil degraders. Strain 15Y was more efficient, it was able to reduce 87.3% of the total petroleum or 99.6% of n-alkanes within the 7-day incubation period at room temperature of 25 ± 2°C.