Publication: Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles
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Issued Date
2008-12-01
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ISSN
03028933
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2-s2.0-56349160516
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Mahidol University
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SCOPUS
Bibliographic Citation
Archives of Microbiology. Vol.190, No.6 (2008), 623-631
Suggested Citation
Veerachat Muangsombut, Supaporn Suparak, Pornpan Pumirat, Suwat Damnin, Paiboon Vattanaviboon, Visith Thongboonkerd, Sunee Korbsrisate Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles. Archives of Microbiology. Vol.190, No.6 (2008), 623-631. doi:10.1007/s00203-008-0413-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18819
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Title
Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles
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Abstract
Burkholderia pseudomallei, an infectious Gram-negative bacterium, is the causative pathogen of melioidosis. In the present study, a B. pseudomallei strain with mutation in the bsaQ gene, encoding a structural component of the type III secretion system (T3SS), was constructed. This bsaQ mutation caused a marked decrease in secretion of BopE effector and BipD translocator proteins into culture supernatant. The B. pseudomallei bsaQ mutant also exhibited decreased efficiencies of plaque formation, invasion into non-phagocytic cells and multinucleated giant cell (MNGC) development in a J774A.1 macrophage cell line. Co-localization of the bacteria and lysosome-associated membrane glycoprotein-1 (LAMP-1) containing vesicles suggested that defects in MNGC formation may result from the delayed ability of this B. pseudomallei mutant to escape from the vacuoles of macrophages. © 2008 Springer-Verlag.