Publication: Inhibition of α-globin gene expression by RNAi
Issued Date
2008-05-09
Resource Type
ISSN
10902104
0006291X
0006291X
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2-s2.0-41149164585
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.369, No.3 (2008), 935-938
Suggested Citation
Orawan Sarakul, Phantip Vattanaviboon, Prapon Wilairat, Suthat Fucharoen, Yasunobu Abe, Koichiro Muta Inhibition of α-globin gene expression by RNAi. Biochemical and Biophysical Research Communications. Vol.369, No.3 (2008), 935-938. doi:10.1016/j.bbrc.2008.02.124 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18920
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Title
Inhibition of α-globin gene expression by RNAi
Abstract
RNA interference (RNAi), a process by which target messenger RNA (mRNA) is cleaved by small interfering complementary RNA (siRNA), is widely used for investigations of regulation of gene expression in various cells. In this study, siRNA complementary to 5′ region of exon II of α-globin mRNA was examined for its role in erythroid colony forming cells (ECFCs) isolated from normal peripheral blood donor. On day 6 of cell culture, 1 × 106ECFCs were transfected with lipofectamine-containing α-globin specific siRNA. After 48 h of transfection, α-globin specific siRNA produced significantly reduction of α-globin mRNA level in a dose-dependent manner, but it did not affect the level of β-globin mRNA. Significantly, decreased numbers of hemoglobinized erythroid cells relative to the control were observed supporting the inhibitory effect of this α-globin mRNA specific siRNA. © 2008 Elsevier Inc. All rights reserved.