Publication: Quantitation of cell-derived microparticles in plasma using flow rate based calibration
1
Issued Date
2008-01-01
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ISSN
01251562
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2-s2.0-39649087812
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.39, No.1 (2008), 146-153
Suggested Citation
Duangdao Nantakomol, Pattamawan Chimma, Nicholas P. Day, Arjen M. Dondorp, Valery Combes, Srivicha Krudsood, Sornchai Looareesuwan, Nicholas J. White, Kovit Pattanapanyasat, Kesinee Chotivanich Quantitation of cell-derived microparticles in plasma using flow rate based calibration. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.39, No.1 (2008), 146-153. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/19843
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Title
Quantitation of cell-derived microparticles in plasma using flow rate based calibration
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Abstract
Activation of vascular endothelium and blood cells can result in the formation of microparticles (MPs), which are membrane vesicles with a diameter < 1 μm which can play a pathogenetic role in a variety of infectious and other diseases. In this study, we validated a modified quantitative method called "flow rate based calibration", to measure circulating MPs in plasma of healthy subjects and malaria patients using FACSCalibur flow cytometry. MPs counts obtained from "flow rate based calibration" correlated closely with the standard method (R2=0.9, p=0.001). The median (range) number of MPs in healthy subjects was 163/μl (81-375/μl). We demonstrated a flow rate based calibration for the quantitation of MPs in P. falciparum malaria-infected patients. The median (range) number of MPs was 2,051/μl (222-6,432/μl), n=28 in patients with falciparum malaria. The number of MPs in plasma from patients with severe falciparum malaria was significantly higher than in uncomplicated falciparum malaria (2,567/μl (366-6,432/μl), n=18 versus [1,947/μl (222-4,107/μl), n=10, p<0.01]. Cellular origin of MPs in malaria patients were mainly derived from red blood cells (35%), platelets (10%), and endothelial cells (5%). There was no significant correlation between the total number of MPs and parasitemia. Flow rate based calibration is a simple, reliable, reproducible method and more affordable to quantitate MPs.