Publication: Biochemical detection of pyrethroid resistance mechanisms in Anopheles minimus in Thailand
3
Issued Date
2003-06-01
Resource Type
ISSN
10811710
Other identifier(s)
2-s2.0-0043209575
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Vector Ecology. Vol.28, No.1 (2003), 108-116
Suggested Citation
Theeraphap Chareonviriyaphap, Pompimol Rongnoparut, Piyanuch Chantarumpom, Michael J. Bangs Biochemical detection of pyrethroid resistance mechanisms in Anopheles minimus in Thailand. Journal of Vector Ecology. Vol.28, No.1 (2003), 108-116. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20633
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Biochemical detection of pyrethroid resistance mechanisms in Anopheles minimus in Thailand
Abstract
Enzyme-based metabolic mechanisms of insecticide resistance were investigated, comparing a deltamethrin-susceptible parent stock and resistant colonies of Anopheles minimus species A using biochemical assays. The control parent colony was determined susceptible to the diagnostic lethal concentration of deltamethrin (0.05%), whereas the 6 resistant test populations at selected 4, 8, 12, 14, 16, and 18 filial generations (F4, F8, F12, F14, F16: and F18) demonstrated varying levels of tolerance/resistance to deltamcthrin. Expression of levels of non-specific esterases, monooxygenases, and glutathione S-transferases (GSTs) were measured. Results indicated that monooxygenase activity was consistently elevated in resistant-selected test populations compared to the parent colony and increased as resistance intensified from F8to F18. There was a 5-fold increase in monooxygenase in the F18generation compared to the parental stock. Fluctuations in alpha and beta-esterase activity, measured by hydrolysis of alpha and beta-naphthylpropionate, provided no conclusive evidence of an association with pyrethroid resistance in this mosquito species. GSTs were not elevated in the 6 resistant test populations. Based on our results, it appears likely that the development of physiological resistance to deltamethrin in laboratory, resistant-selected generations of An. minimus is primarily associated with increased detoxification by over-expression of monooxygenases. The oxidases are the major contributors to pyrethroid resistance and the importance of kdr has yet to be convincingly determined. This finding represents the first report from Thailand of this metabolic mechanism of resistance in anophelines.