This study was focused on establishment of piezoelectric biosensor for direct detection of
Mycobacterium tuberculosis (MTB) in clinical specimens. The quartz crystal immobilized via
3-mercaptopropionic acid (MPA)/avidin/DNA biotinylated probe on gold surface and hybridization of
the DNA target to DNA biotinylated probe. The optimal concentration of MPA, avidin and
5’-biotinylated DNA probe for immobilization of specific DNA probe on gold surface were 15 mM,
0.1 mg/ml and 1.5 μM, respectively. The detection of genomic DNA digestion in the range from 0.5 to
30 μg/ml. The fabricated biosensor was evaluated through an examination of 200 samples. No cross
hybridization were observed against M. avium complex (MAC) and other microorganism. This target
DNA preparation without amplification will reduce time consuming, costs, and the tedious step of
amplification. This study can be extended to develop the new method which is high sensitivity,
specificity, cheap, easy to use, and rapid for detection of MTB in many fields.
