Publication: Effects of D<inf>1</inf>- and D<inf>2</inf>-dopamine receptor activation on melatonin synthesis in bovine pinealocytes
Issued Date
2003-10-01
Resource Type
ISSN
07423098
Other identifier(s)
2-s2.0-0042913140
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Pineal Research. Vol.35, No.3 (2003), 169-176
Suggested Citation
Chorthip Santanavanich, Banthit Chetsawang, Manuchair Ebadi, Piyarat Govitrapong Effects of D<inf>1</inf>- and D<inf>2</inf>-dopamine receptor activation on melatonin synthesis in bovine pinealocytes. Journal of Pineal Research. Vol.35, No.3 (2003), 169-176. doi:10.1034/j.1600-079X.2003.00073.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20690
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Title
Effects of D<inf>1</inf>- and D<inf>2</inf>-dopamine receptor activation on melatonin synthesis in bovine pinealocytes
Abstract
Previous studies have identified and characterized D1- and D2-dopamine receptors in bovine pineal glands. The data indicate that the density of D1-dopamine receptors (974 fmol/mg protein) far exceed that of D2-dopamine receptors (37 fmol/mg protein). The objective of this study was to identify the mRNAs for both D1- and D2-dopamine receptors and to elucidate the status of dopamine and its possible involvement in the pineal function, particularly on melatonin synthesis. The expression of these dopamine receptor subtypes were determined by using a reverse transcriptase-polymerase chain reaction technique with specific pairs of primers to amplify D1- and D2-dopamine receptor mRNAs. Amplification of RNAs from bovine striatum (positive control) and bovine pineal gland resulted in products of the predicted lengths of 231 bp for D1- and 333 bp for D2-dopamine receptors. The results indicate that both D1- and D2-dopamine receptor mRNAs are present in the bovine pineal gland. The role of dopamine receptors was investigated by studying the effects of selective D1- and D2-dopamine agonists and antagonists on the N-acetyltransferase (NAT) activity of cultured bovine pinealocytes. The data showed that SKF-38393, a selective D1-agonist, enhanced NAT activity, and increased melatonin level, and the stimulatory effect was blocked by SCH-23390, a D1-selective antagonist, whereas quinpirole, a selective D2-agonist, inhibited NAT basal activity and decreased the melatonin basal level. Furthermore the inhibitory effect was blocked by D2-selective antagonists, spiperone, haloperidol, and domperidone. The present results indicate that the pineal dopamine receptors have a distinct effect on pineal function. The precise mechanism whereby activation of dopamine receptors altered the NAT activity and melatonin level needs to be further delineated.