Publication: Phosphatidylserine exposure in Fas type I cells is mitochondria-dependent
Issued Date
2003-06-19
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ISSN
00145793
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2-s2.0-0038202902
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Mahidol University
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SCOPUS
Bibliographic Citation
FEBS Letters. Vol.545, No.2-3 (2003), 110-114
Suggested Citation
Wanlaya Uthaisang, Leta K. Nutt, Sten Orrenius, Bengt Fadeel Phosphatidylserine exposure in Fas type I cells is mitochondria-dependent. FEBS Letters. Vol.545, No.2-3 (2003), 110-114. doi:10.1016/S0014-5793(03)00508-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20718
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Title
Phosphatidylserine exposure in Fas type I cells is mitochondria-dependent
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Abstract
Previous studies have demonstrated that Fas-triggered activation of effector caspases and subsequent nuclear apoptosis either is mitochondria-independent (type I cells) or relies on mitochondrial amplification of the initial stimulus (type II cells). We show herein that Bcl-2 overexpression in a prototypic type I cell line (SKW6.4) promotes mitochondrial generation of ATP and blocks Fas-triggered plasma membrane externalization of phosphatidylserine (PS). Moreover, overexpression of Bcl-2 attenuates macrophage engulfment of Fas-triggered cells. Fas-mediated DNA fragmentation, on the other hand, remains unaffected in SKW6.4-bcl-2 cells. These studies thus demonstrate that PS externalization and clearance of cell corpses are mitochondria-dependent events, and show that these events can be dissociated from other features of the apoptotic program, in Fas type I cells. © 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.