Publication: Transglucosylation of tertiary alcohols using cassava β-glucosidase
Issued Date
2003-06-06
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0006291X
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2-s2.0-0037902560
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.305, No.3 (2003), 470-475
Suggested Citation
Jisnuson Svasti, Thanawat Phongsak, Rakrudee Sarnthima Transglucosylation of tertiary alcohols using cassava β-glucosidase. Biochemical and Biophysical Research Communications. Vol.305, No.3 (2003), 470-475. doi:10.1016/S0006-291X(03)00793-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20719
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Title
Transglucosylation of tertiary alcohols using cassava β-glucosidase
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Abstract
We have compared the ability of β-glucosidases from cassava, Thai rosewood, and almond to synthesize alkyl glucosides by transglucosylating alkyl alcohols of chain length C1-C8. Cassava linamarase shows greater ability to transfer glucose from p-nitrophenyl-β-glucoside to secondary alcohol acceptors than other β-glucosidases, and is unique in being able to synthesize C4, C5, and C6tertiary alkyl β-glucosides with high yields of 94%, 82%, and 56%, respectively. Yields of alkyl glucosides could be optimized by selecting appropriate enzyme concentrations and incubation times. Cassava linamarase required pNP-glycosides as donors and could not use mono- or di-saccharides as sugar donors in alkyl glucoside synthesis. © 2003 Elsevier Science (USA). All rights reserved.