Publication: Behavior of the Dengue Virus in Solution
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Issued Date
2003-12-01
Resource Type
ISSN
01466615
Other identifier(s)
2-s2.0-0142244382
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Medical Virology. Vol.71, No.4 (2003), 532-539
Suggested Citation
Patchima Sithisarn, Lukkana Suksanpaisan, Chutima Thepparit, Duncan R. Smith Behavior of the Dengue Virus in Solution. Journal of Medical Virology. Vol.71, No.4 (2003), 532-539. doi:10.1002/jmv.10520 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20858
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Title
Behavior of the Dengue Virus in Solution
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Abstract
The dengue virus consists of four antigenically related but distinct viruses, termed Dengue virus 1-4 (DEN 1-4). We have established that the dengue virus loses infectivity over time in solution in an exponentially declining manner. The four strains examined (one from each serotype) have half-lives that range from 2.5 to 7.5 hr in defined medium. The half-life is temperature and pH-dependent and is affected by the nature of the host cell in which it is produced, but is not dependent upon the presence of either Mg2+ ions or chelating agents. Electron microscopy (EM) of solutions of the dengue virus show almost complete virus aggregation after 24 hr at room temperature, while RT-PCR shows an intact RNA genome. These results show that the solution environment of the dengue virus is an important determinant of dengue virus infectivity. © 2003 Wiley-Liss, Inc.