Publication: Low-cost CD4 enumeration in HIV-infected patients in Thailand
1
Issued Date
2003-06-01
Resource Type
ISSN
0125877X
Other identifier(s)
2-s2.0-0242610778
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.21, No.2 (2003), 105-113
Suggested Citation
Kovit Pattanapanyasat, Surada Lerdwana, Hla Shain, Egarit Noulsri, Charin Thepthai, Varipin Prasertsilpa, Achara Eksaengsri, Krisana Kraisintu Low-cost CD4 enumeration in HIV-infected patients in Thailand. Asian Pacific Journal of Allergy and Immunology. Vol.21, No.2 (2003), 105-113. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20899
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Low-cost CD4 enumeration in HIV-infected patients in Thailand
Other Contributor(s)
Abstract
In Thailand, over one million people have been infected with HIV since the beginning of the epidemic. This has created a great burden on the country's limited health care budget. Monitoring CD4+ T-lymphocytes is important to determine the success of any antiretroviral therapy as well as HIV vaccine trials. However, the high cost of CD4 counts makes monitoring of every HIV-infected patient impossible in Thailand. Therefore, the development of affordable strategies is necessary in order to allow more HIV infected persons to access CD4 testing to control the disease. The current standard methods for enumeration of CD4+ T-lymphocytes are performed on whole blood by flow cytometric immunophenotyping using the 6-tube 2-color and 3-tube 3-color panels recommended by the Centers for Diseases Control (CDC). In this study, percentage CD4+ T-lymphocyte values (from 142 HIV-seropositive patients and 26 anti-HIV negative adult blood donors) generated by the use of just 2 reagents (CD45/CD4) in a 1-tube 2-color panel employing side scatter/CD45 morphospectral gating were compared to those obtained by state of the art methods. We also compared the use of generic monoclonal antibody reagents with commercial reagents and found the results to be comparable with an overall correlation coefficient (r) of more than 0.95 for both CD4+ and CD8+ T-lymphocytes. Bland-Altman analysis of the mean CD4 values plotted against the difference in values between the generic reagents and the commercial reagents showed no bias. The 1-tube 2-color method using generic monoclonal antibody reagents potentially permits more affordable but reliable CD4 testing and therefore could increase access for more HIV-infected patients in resource-poor countries.