Publication: The use of flow cytometry as a diagnostic test for malaria parasites
1
Issued Date
2004-09-01
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ISSN
01251562
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2-s2.0-20844445789
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.35, No.3 (2004), 552-559
Suggested Citation
Varee Wongchotigul, Nirut Suwanna, Srivicha Krudsood, Duangrudee Chindanond, Shigeyuki Kano, Nobuaki Hanaoka, Yasumasa Akai, Yasunori Maekawa, Satoshi Nakayama, Somei Kojima, Sornchai Looareesuwan The use of flow cytometry as a diagnostic test for malaria parasites. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.35, No.3 (2004), 552-559. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21577
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Title
The use of flow cytometry as a diagnostic test for malaria parasites
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Abstract
A total of 453 clinical blood samples were determined for malaria parasites by flow cytometric assay (FCM) and reagents from Sysmex Corporation, Japan. In this study, the FCM greatly simplified and accelerated parasite detection, with sensitivity of 91.26%, specificity 86.28% and accuracy 87.42%. Overall, the parasite counts by flow cytometric measurement correlated well with the parasitemia measured by microscopic assay (regression coefficient = 0.9409). The detection limit was 0.05-0.1% parasitemia. No evidence of malaria parasites in either blood donor volunteers or other disease patients groups was determined by FCM. However, 48 samples who had been treated with antimalarial drugs and whose parasite microscopic counts were negative, showed false-positive results. When the data of these 48 samples were analyzed, they were found to have high levels of reticulocytes, ranging from 2.0-18.9%. This finding suggested that a high reticulocyte concentration in the blood may interfere with the performance of the FCM. Further improvement, by eliminating this interference, will make the FCM one of the most promising tests for malaria diagnosis.