Publication: Isolation and characterization of Opisthorchis viverrini specific DNA probe
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Issued Date
1991-01-01
Resource Type
ISSN
08908508
Other identifier(s)
2-s2.0-0026263952
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular and Cellular Probes. Vol.5, No.6 (1991), 399-407
Suggested Citation
Rasana Sermswan, Skorn Mongkolsuk, Sakol Panyim, Stitaya Sirisinha Isolation and characterization of Opisthorchis viverrini specific DNA probe. Molecular and Cellular Probes. Vol.5, No.6 (1991), 399-407. doi:10.1016/S0890-8508(05)80011-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21996
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Title
Isolation and characterization of Opisthorchis viverrini specific DNA probe
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Abstract
An Opisthorchis viverrini specific repetitive DNA fragment was isolated from the O. viverrini genome. The cloned fragment, herein designated pOV-A6, contained tandemly repeated 334 bp O. viverrini DNA and has the potential to be a highly specific and sensitive DNA probe. Radioactively labelled pOV-A6 could detect as little as 25 pg of its own genomic DNA and five purified eggs by dot-blot hybridization. The presence of 50 O. viverrini eggs could be readily detected in samples containing faecal materials. The probe was highly specific for O. viverrini DNA and did not cross hybridize with DNAs prepared from related and unrelated parasites, enteric bacteria and a number of definitive and intermediate hosts. The probe has potential for screening a large number of samples and could be readily applied in epidemiological studies. © 1991 Academic Press Ltd.