Publication: Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon
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Issued Date
2006-12-11
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ISSN
00448486
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2-s2.0-33751320634
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Mahidol University
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SCOPUS
Bibliographic Citation
Aquaculture. Vol.261, No.4 (2006), 1447-1454
Suggested Citation
Kanokpan Wongprasert, Somluk Asuvapongpatana, Pisit Poltana, Montip Tiensuwan, Boonsirm Withyachumnarnkul Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon. Aquaculture. Vol.261, No.4 (2006), 1447-1454. doi:10.1016/j.aquaculture.2006.08.044 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/22851
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Title
Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon
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Abstract
Serotonin (5-hydroxytryptamine, 5HT) has been reported to induce ovarian maturation and spawning in the crayfish Procambarus clarkii and white Pacific shrimp Litopenaeus vannamei. The aim of this study was to explore the role of exogenous 5HT on the reproductive performance of the black tiger shrimp Penaeus monodon. 5HT solution was injected into domesticated P. monodon broodstock at 50 μg/g body weight and ovarian maturation and spawning were recorded. The presence of 5HT in the ovary and oviduct of P. monodon was also studied by immunohistochemistry and its levels in the ovary by enzyme link immunoabsorbance assay (ELISA). The 5HT-injected P. monodon developed ovarian maturation and spawning rate at the level comparable to that of unilateral eyestalk-ablated shrimp. Hatching rate and the amount of nauplii produced per spawner were also significantly higher in the 5HT-injected shrimp, compared to the eyestalk-ablated shrimp. 5HT-positive reactions were found in the follicular cells of pre-vitellogenic oocytes, in the cytoplasm of early vitellogenic oocytes and on the cell membrane and cytoplasm of late vitellogenic oocytes. 5HT in the ovary was present at 3.53 ± 0.26 ng/mg protein level in previtellogenic stage and increased to 17.03 ± 0.57 ng/mg protein level in the mature stage of the ovary. The results suggest a significant role of 5HT, possibly directly on the ovary and oviduct, on the reproductive function of female P. monodon. © 2006 Elsevier B.V. All rights reserved.